Cross-Talk in Nucleotide Signaling in Glioma C6 Cells

摘要: The chapter is focused on the mechanism of action metabotropic P2Y nucleotide receptors: P2Y1, P2Y2, P2Y12, P2Y14 and ionotropic P2X7 receptor in glioma C6 cells. P2Y1 P2Y12 both respond to ADP, but while links PLC elevates cytosolic Ca2+ concentration, negatively couples adenylate cyclase, maintaining cAMP at low level. In C6, these two receptors modulate activities ERK1/2 PI3K/Akt signaling effects depend physiological conditions During prolonged serum deprivation, cell growth arrested, expression strongly decreases becomes a major player responsible for ADP-evoked signal transduction. activates kinase phosphorylation (a known proliferation regulator) stimulates Akt activity, contributing invasiveness. contrast, has an inhibitory effect pathway signaling. Furthermore, receptor, often apoptotic fate, not involved Ca2+elevation shift from during withdrawal, cross talk between lack activity shows precise self-regulating mechanism, enhancing survival preserving neoplastic features