Azotobactervinelandii RNA polymerase VI. An in situ assay for polymerase after acrylamide gel electrophoresis

摘要: Abstract Analysis of the A. vinelandii RNA polymerase by acrylamide gel electrophoresis indicated that enzyme activity was associated with more than one resolved protein bands. Initially determined after elution from gels sliced into discs ( Chrambach, 1966 ). However, recovery variable and closely spaced bands could not be consistently assayed separately. The present paper describes a procedure for assaying in gel. This assay utilizes unprimed synthesis poly A·poly U Smith et al, 1967 ; Krakow, 1968 ) or rIC copolymer Krakow Karstadt, );followed staining ethidium bromide to visualize polymers.