Comparative Characterization of Glutamate Decarboxylase in Crude Homogenates of Oviduct, Ovary, and Hypothalamus

摘要: Some biochemical characteristics of L-glutamate decarboxylase (GAD) were compared using crude homogenates the rat oviduct, ovary, and hypothalamus. As estimated by measurement CO2 production, specific activities oviductal ovarian GAD about 10 3% hypothalamic value, respectively. Stoichiometric formation gamma-aminobutyric acid (GABA) from could be observed in oviduct hypothalamus, while production was more than nine times that GABA. Hypothalamic tubal showed similar time course, temperature dependence, pH dependence. The dependence on protein concentration exogenous cofactor supply also these two tissues. kinetic constant for as a substrate nearly same (1.30 mM) hypothalamus (1.64 mM). responsiveness to various inhibitors similar. present findings indicate may identical, they suggest possible GABAergic innervation Fallopian tube.