High Specific γ‐Aminobutyric Acid Binding to Membranes of the Human Ovary
作者： Sándor L. Erdö , Ádám László
DOI: 10.1111/J.1471-4159.1984.TB02810.X
关键词: Picrotoxin 、 Amino acid 、 Muscimol 、 Internal medicine 、 Endocrinology 、 Biology 、 Aminobutyric acid 、 GABAA receptor 、 Bicuculline 、 Population 、 Ovary
摘要: The concentration of gamma-aminobutyric acid (GABA) in the human ovary and capacity a membrane preparation from same organ to bind [3H]GABA specifically were examined. GABA was found be 214 +/- 66 nmol/g frozen tissue (mean SEM six independent determinations). Moreover, single population high-affinity binding sites has been identified ovarian membranes. apparent dissociation constant (Kd) maximum (Bmax) 38.3 nM 676 fmol/mg protein, respectively. specific displaced by muscimol, unlabelled GABA, or (+)bicuculline, but unaffected (+/-)baclofen picrotoxin. present results show that an extremely high density GABAA receptor are ovary, indicating physiological significance this amino female reproductive system.
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