Binding of [3H]-muscimol to GABAA sites in the guinea-pig urinary bladder: biochemical assay and autoradiography.
作者： S.L. Erdö , M.C. Mione , F. Amenta , J.R. Wolff
DOI: 10.1111/J.1476-5381.1989.TB11819.X
关键词: Muscimol 、 Bladder Fundus 、 Whole membrane 、 Binding site 、 Internal medicine 、 gamma-Aminobutyric acid 、 Urinary bladder 、 Endocrinology 、 Bicuculline 、 GABAA receptor 、 Biophysics 、 Chemistry
摘要: 1. The specific binding of [3H]-muscimol, a gamma-aminobutyric acidA receptor (GABAA) agonist, to whole membranes the guinea-pig urinary bladder was examined. In addition, distribution muscimol sites within visualized by autoradiography. 2. It demonstrated that in frozen-thawed membrane preparation organ [3H]-muscimol is reproducible, reversible and saturable. 3. Saturable single component with an equilibrium dissociation constant (Kd) 12 nM maximal density (Bmax) about 80 fmol mg-1 protein. 4. displacement experiments several model compounds, showed characteristics GABAA site. 5. Autoradiographic revealed uneven specifically bound bladder. high clusters smooth muscle layers fundus urethra, while apex neck were not labelled. 6. present findings show type may be labelled reproducible manner. Moreover, localization these consistent presence receptors only subpopulation vesical ganglia.
sci-hub.se 参考文章(21)
G. Y. Minuk, C. E. Bear, E. J. Sarjeant, Sodium-independent, bicuculline-sensitive [3H]GABA binding to isolated rat hepatocytes. American Journal of Physiology-gastrointestinal and Liver Physiology. ,vol. 252, ,(1987) , 10.1152/AJPGI.1987.252.5.G642
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Sándor L. Erdö, Ádám László, High Specific γ‐Aminobutyric Acid Binding to Membranes of the Human Ovary Journal of Neurochemistry. ,vol. 42, pp. 1464- 1467 ,(1984) , 10.1111/J.1471-4159.1984.TB02810.X
Y Kataoka, Y Gutman, A Guidotti, P Panula, J Wroblewski, D Cosenza-Murphy, JY Wu, E Costa, None, Intrinsic GABAergic system of adrenal chromaffin cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 3218- 3222 ,(1984) , 10.1073/PNAS.81.10.3218
Diana N. Krause, Esther Wong, Phyllis Degener, Eugene Roberts, GABA receptors in bovine cerebral blood vessels: Binding studies with [3H]muscimol Brain Research. ,vol. 185, pp. 51- 57 ,(1980) , 10.1016/0006-8993(80)90669-1
P. Martinez, A. Gimenez, E. Castro, M.J. Oset-Gasque, S. Cañadas, M.P. González, GABA binding in bovine adrenal medulla membranes is sensitive to baclofen. Comparative Biochemistry and Physiology Part C: Comparative Pharmacology. ,vol. 88, pp. 155- 157 ,(1987) , 10.1016/0742-8413(87)90060-0
Sándor L. Erdö, Erzsébet Lapis, Bicuculline-sensitive GABA receptors in rat ovary European Journal of Pharmacology. ,vol. 85, pp. 243- 246 ,(1982) , 10.1016/0014-2999(82)90475-7
B. Reusens-Billen, X. Pirlot, C. Remacle, J. J. Hoet, M. de Gasparo, Localization of Gaba High-affinity Binding-sites in the Pancreas of Neonatal Rat Cell and Tissue Research. ,vol. 235, pp. 503- 508 ,(1984) , 10.1007/BF00226946
Sándor L. Erdö, Erzsébet Lapis, Presence of GABA receptors in rat oviduct Neuroscience Letters. ,vol. 33, pp. 275- 279 ,(1982) , 10.1016/0304-3940(82)90384-6
Paolo Napoleone, Sándor Erdö, Francesco Amenta, Autoradiographic localization of the GABAA receptor agonist [3H]muscimol in rat cerebral vessels Brain Research. ,vol. 423, pp. 109- 115 ,(1987) , 10.1016/0006-8993(87)90830-4