UVB irradiation as a tool to assess ROS-induced damage in human spermatozoa

摘要: One of the consequences oxygen metabolism is production reactive species (ROS) which in a situation imbalance with antioxidants can damage several biomolecules, compromise cell function and even lead to cellular death. The particularities sperm make it particularly vulnerable ROS attack compromising its functionality, mirrored terms fertility outcome making study origin ROS, as well alterations they cause very important. In present work, we used UVB irradiation, an easy experimental approach known potent inducer formation, better understand without any confounding effects that usually exist disease models are reported play role. To address these issues evaluated mitochondrial using Mitosox Red Probe, membrane potential JC-1 probe, lipid peroxidation through BODIPY probe vitality PI. We observed irradiation leads increase occur previously observable dysfunction. concluded appears be good easily manipulated vitro model system mitochondria-induced oxidative stress spermatozoa consequences, may relevant dissecting action pathways many other pathologies, drugs contaminants, including endocrine disruptors.