MicroRNAs Induced During Ischemic Preconditioning

摘要: Background and Purpose— MicroRNAs (miRNA) are single-stranded short RNA molecules that regulate gene expression by either degradation or translational repression of mRNA. Although miRNAs control a number conditions diseases, few neuroprotective have been described. In this study, we investigated induced early in ischemic preconditioning. Methods— Ischemic preconditioning focal cerebral ischemia was mice transient occlusion the middle artery for 15 120 minutes. We prepared samples from cortex at 3 24 hours after onset ischemia. Selective then were synthesized transfected into Neuro-2a cells before oxygen–glucose deprivation. Results— detected total 360 miRNAs. Two miRNA families, miR-200 miR-182, selectively upregulated Transfections some these vitro Among them, miR-200b, miR-200c, miR-429 target...