Agglomeration of Escherichia coli with Positively Charged Nanoparticles Can Lead to Artifacts in a Standard Caenorhabditis elegans Toxicity Assay

摘要: The increased use and incorporation of engineered nanoparticles (ENPs) in consumer products requires a robust assessment their potential environmental implications. However, lack standardized methods for nanotoxicity testing has yielded results that are sometimes contradictory. Standard ecotoxicity assays may work appropriately some ENPs with minimal modification but produce artifactual others. Therefore, understanding the robustness range is critical. In this study, we evaluated performance standard Caenorhabditis elegans (C. elegans) toxicity assay containing an Escherichia coli (E. coli) food supply silicon, polystyrene, gold different charged coatings sizes. Of all tested, only those positively coating caused growth inhibition. were observed to heteroagglomerate E. coli cells, suggesting impacted ability nematodes feed, leading f...