NK- and CD8(+) T cell-mediated eradication of established tumors by peritumoral injection of CpG-containing oligodeoxynucleotides.

摘要: Unmethylated cytosine-phosphorothioate-guanine (CpG) containing oligodeoxynucleotides (CpG-ODN) are known to act as adjuvants and powerful activators of the innate immune system. We investigated therapeutic effect CpG-ODN on a variety established mouse tumors including AG104A, IE7 fibrosarcoma, B16 melanoma, 3LL lung carcinoma. These only weakly immunogenic notoriously difficult treat. Repeated peritumoral injection resulted in complete rejection or strong inhibition tumor growth, whereas systemic application had partial effects. The CpG-ODN-induced was found be mediated by both NK tumor-specific CD8 + T cells. Comparison parental variants rendered more antigenic transfection with Ags suggested that efficiency therapy correlated antigenicity tumors. Peritumoral treatment even effective situation where system tolerant for Ag, shown breakage tolerance elimination. results suggest acts locally inducing cells, also leads efficient presentation stimulation effector memory thus providing antitumor can applied without knowledge Ag.