Efficient Proliferation and Maturation of Fetal Liver Cells in Three-Dimensional Culture by Stimulation of Oncostatin M, Epidermal Growth Factor, and Dimethyl Sulfoxide
作者： Toshie Koyama , Tomo Ehashi , Norio Ohshima , Hirotoshi Miyoshi
DOI: 10.1089/TEN.TEA.2008.0242
关键词:
摘要: For the purpose of applying fetal liver cells (FLCs) as a cell source to tissue-engineered bioartificial livers, three-dimensional (3-D) cultures FLCs using porous polymer scaffold, well monolayer control, were simultaneously performed. To achieve efficient growth and differentiation, cultured in medium for first 3 weeks then differentiation more weeks. In these cultures, stimulating factors (oncostatin M (OSM), epidermal factor (EGF), hepatocyte (HGF), or dimethyl sulfoxide (DMSO)) added media, their effects examined. When containing OSM EGF was used, stimulated synergistically with OSM. into mature hepatocytes, DMSO remarkably enhanced albumin secretion 3-D although HGF effective only culture. Microscopic observati...
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