AML1-ETO9a is correlated with C-KIT overexpression/mutations and indicates poor disease outcome in t(8;21) acute myeloid leukemia-M2

摘要: AML1-ETO fusion gene is generated from chromosomal translocation t(8;21) mainly in acute myeloid leukemia M2 subtype (AML-M2). Its spliced variant transcript, AML1-ETO9a, rapidly induces murine model. To evaluate its clinical significance, AML1-ETO9a expression was assessed 118 patients with AML-M2, using qualitative and nested quantitative reverse transcriptase (RT)-PCR methods. These cases were accordingly divided into the AML1-ETO9a-H group (n=86, positive for RT-PCR, higher level of by RT-PCR) AML1-ETO9a-L (n=32, negative lower but still detectable RT-PCR). C-KIT significantly increased group, as compared group. Of 36 harboring mutations, 32 overexpressed (P=0.0209). Clinically, exhibited elevated white blood cells count, less bone marrow aberrant myelocytes, CD56 decreased CD19 (P=0.0451, P=0.0479, P=0.0149 P=0.0298, respectively). Moreover, overexpression related to short event-free overall survival time (P=0.0072 P=0.0076, Taken together, these data suggest that correlated overexpression/mutations indicates poor disease outcome AML-M2.