Analysis of the role of AML1-ETO in leukemogenesis, using an inducible transgenic mouse model

摘要: As reported previously, AML1-ETO knock-in mice were generated to investigate the role of in leukemogenesis and mimic progression t(8;21) leukemia. These died midgestation because hemorrhaging central nervous system a block definitive hematopoiesis during embryogenesis. Therefore, they are not good model for development acute myeloid which expression is under control tetracycline-inducible system. Multiple lines transgenic have been produced with complementary DNA controlled by tetracycline-responsive element. In absence antibiotic tetracycline, strongly expressed bone marrow tet-controlled transcriptional activator double-positive mice. Furthermore, addition tetracycline reduces nondetectable levels. Throughout normal murine lifespan 24 months, expressing developed spite this, abnormal maturation proliferation progenitor cells observed from these animals. results demonstrate that has very restricted capacity transform cells. Either introduction additional genetic changes or at particular stage hematopoietic cell differentiation will be necessary develop studying pathogenesis t(8;21).