Chemoenzymatic approaches to the dynamic kinetic asymmetric synthesis of aromatic amino acids

摘要: Abstract Enzymatic approaches for the production of amino acids by nitrilases are described. Dynamic kinetic asymmetric synthesis conditions were established aromatic aminonitriles, phenylglycinonitrile and 4-fluorophenylglycinonitrile, at high pH to produce corresponding acid products in enantiomeric excess. N -Acylation aminonitriles led spontaneous racemization pH 8, allowing preferential enzymatic hydrolysis ( R )-enantiomer afford product -acylamino up 99% excess (ee).