Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines

摘要: A central question in neuronal network analysis is how the interaction between individual neurons produces behavior and behavioral modifications. This task depends critically on exactly signals are integrated by nerve cells functioning as complex operational units. Regional electrical properties of branching processes which determine input-output function any neuron extraordinarily complex, dynamic, and, general case, impossible to predict absence detailed measurements. To obtain such a measurement one would, ideally, like be able monitor, at multiple sites, subthreshold events they travel from sites origin (synaptic contacts distal dendrites) summate particular locations influence action potential initiation. It became possible recently carry out this type using high-resolution multisite recording membrane changes with intracellular voltage-sensitive dyes. chapter reviews development foundation method dye neurons. Presently, approach allows monitoring transients all parts dendritic tree well axon collaterals spines.