Tracking individual action potentials throughout mammalian axonal arbors.

摘要: Axons are neuronal processes specialized for conduction of action potentials (APs). The timing and temporal precision APs when they reach each the synapses fundamentally important information processing in brain. Due to small diameters axons, direct recording single AP transmission is challenging. Consequently, most knowledge about axonal conductance derives from modeling studies or indirect measurements. We demonstrate a method noninvasively directly record individual propagating along millimeter-length arbors cortical cultures with hundreds microelectrodes at microsecond resolution. find that axons conduct high (~100 µs arrival time jitter per mm length) reliability: more than 8,000,000 recorded APs, we did not observe any branch-point failures. Upon high-frequency stimulation 100 Hz, successive became slower, their decreased by 20% 12% 100th AP, respectively.