Reprogramming the translation initiation for the synthesis of physiologically stable cyclic peptides.

摘要: The initiation codon dictates that the translation event exclusively begins with methionine. We report here a new technology to reprogram event, where various amino acids and those bearing Nα-acyl groups can be used as an initiator for peptide synthesis. is built upon concept of genetic code reprogramming, methionine depleted from system reassigned desired acid. have applied this synthesis antitumor cyclic peptide, G7−18NATE, closed by physiologically stable bond, it also extended custom its analogues ring sizes. Significantly, cyclization occurs spontaneously precursor linear peptides. To demonstrate practicality methodology, we prepared small library designated 160 distinct mRNAs. Thus, offers means prepare wide array in vivo compatible ...