[1] Peptide thioester substrates for serine peptidases and metalloendopeptidases
作者： James C. Powers , Chih-Min Kam
DOI: 10.1016/0076-6879(95)48003-X
关键词:
摘要: Abstract Peptide thioesters are sensitive substrates of various serine peptidases and metalloendopeptidases. Thioester generally have high enzymatic hydrolysis rates low background rates, the can be easily monitored in presence thiol reagents such as 4,4′-dithiodipyridine or 5,5′-dithiobis (2-nitrobenzoic acid). thioester been invaluable for study enzyme specificity inhibitors, especially cases where no other practical synthetic available. Tripeptide type Boc-Ala-Ala-AA-SBzl, AA is nearly all 20 common amino acids, now synthesized should useful subsite mapping new crude cell preparations containing peptidases.
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