Effects of furfural and acetic acid on growth and lipid production from glucose and xylose by Rhodotorula glutinis

作者: Guochang Zhang , William Todd French , Rafael Hernandez , Earl Alley , Maria Paraschivescu

DOI: 10.1016/J.BIOMBIOE.2010.10.009

关键词:

摘要: Microbial conversion of lignocellulosic sugars to triacylglycerols (a biodiesel or renewable diesel feedstock) was investigated using the oleaginous yeast Rhodotorula glutinis (ATCC 15125). In shake flask experiments, R. first grown in a nitrogen-rich medium utilizing an artificial acid hydrolysate biomass switchgrass as sole carbon and energy source. Once culture had reached stationary phase, cells were harvested transferred fresh nitrogen-free media containing for lipid accumulation. Analysis data collected showed that able grow The net specific Growth rate(s) indicated presence acetic furfural inhibited growth on glucose, but not xylose. accumulated cells, determined by gravimetrical method, increased from initial 4.3%–39.0% dry cell mass weight. major fatty acids lipids palmitic acid, stearic oleic linoleic γ-linoleic acid. These results indicate it is feasible convert glutinis.

参考文章(18)
Nathan Mosier, Charles Wyman, Bruce Dale, Richard Elander, YY Lee, Mark Holtzapple, Michael Ladisch, Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresource Technology. ,vol. 96, pp. 673- 686 ,(2005) , 10.1016/J.BIORTECH.2004.06.025
Suniti Misra, Amitabha Ghosh, Jyotirmoy Dutta, Production and composition of microbial fat fromRhodotorula glutinis Journal of the Science of Food and Agriculture. ,vol. 35, pp. 59- 65 ,(1984) , 10.1002/JSFA.2740350110
E. G. Bligh, W. J. Dyer, A rapid method of total lipid extraction and purification. Biochemistry and Cell Biology. ,vol. 37, pp. 911- 917 ,(1959) , 10.1139/O59-099
Gerhard Knothe, Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters Fuel Processing Technology. ,vol. 86, pp. 1059- 1070 ,(2005) , 10.1016/J.FUPROC.2004.11.002
Jacek Leman, Oleaginous microorganisms: an assessment of the potential. Advances in Applied Microbiology. ,vol. 43, pp. 195- 243 ,(1997) , 10.1016/S0065-2164(08)70226-0
Cheng S. Gong, Recent Advances in D-Xylose Conversion by Yeasts Annual Reports on Fermentation Processes. ,vol. 6, pp. 253- 297 ,(1983) , 10.1016/B978-0-12-040306-6.50015-8
R.R. Bakker, J.H.O. Hazewinkel, R.H.W. Maas, T. de Vrije, R.J.A. Gosselink, E. de Jong, J.W. van Groenestijn, BIOFUEL PRODUCTION FROM ACID-IMPREGNATED WILLOW AND SWITCHGRASS S.n.. pp. 1467- 1470 ,(2004)
Colin Ratledge, James P. Wynn, The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms Advances in Applied Microbiology. ,vol. 51, pp. 1- 51 ,(2002) , 10.1016/S0065-2164(02)51000-5
Simona Larsson, Eva Palmqvist, Bärbel Hahn-Hägerdal, Charlotte Tengborg, Kerstin Stenberg, Guido Zacchi, Nils-Olof Nilvebrant, The generation of fermentation inhibitors during dilute acid hydrolysis of softwood Enzyme and Microbial Technology. ,vol. 24, pp. 151- 159 ,(1999) , 10.1016/S0141-0229(98)00101-X
Caidian Luo, David L. Brink, Harvey W. Blanch, Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanol Biomass and Bioenergy. ,vol. 22, pp. 125- 138 ,(2002) , 10.1016/S0961-9534(01)00061-7