Inactivation of Escherichia coli O157:H7 in vitro and on the surface of spinach leaves by biobased antimicrobial surfactants

摘要: This study was conducted to evaluate the effect of biosurfactants on populations Escherichia coli O157:H7 in suspension and spinach leaves. Eight surfactants including four soybean oil-based biosurfactants, sodium dodecyl sulfate (SDS), polyoxyethylene sorbitan monooleate (Tween 80), sophorolipid (SO) thiamine dilauryl (TDS) at concentrations 0.1%, 0.5% 1.0% were tested bacterial suspension, most effective applied Results showed that SDS or Tween 80 did not significantly affect E. coli populations. SO TDS reducing suspension. with an initial population 7.1 log CFU/mL detectable (detection limit: 1 CFU/mL) after 1 min 2 h SO. On leaves, 1% when compared a water wash during 7 days post-treatment storage 4 °C. However, (1.0%) as 200 ppm chlorine spot inoculated O157:H7, achieving 3.1 2.7 CFU/per leaf day 0, 1.4 1.9 CFU/leaf wash. No apparent change visual quality observed. None treatments caused changes spinach. Electron micrographs suggested ultrastructural damage cells such separation outer membrane from cytoplasmic membrane. Overall, our results may be potential sanitizers inactivating human pathogens fresh produce.