Selective breeding for desiccation tolerance in liquid culture provides genetically stable inbred lines of the entomopathogenic nematode Heterorhabditis bacteriophora.

摘要: The entomopathogenic nematode (EPN) Heterorhabditis bacteriophora is used in biological plant protection to control pest insects. In the past, several attempts targeted at an enhancement of desiccation tolerance EPN by genetic selection order improve their storage stability. subsequent loss improved beneficial traits after release pressure has often been reported. stabilize progress selective breeding, during liquid culturing was tested against propagation host After pressure, monitored over additional reproductive cycles vivo and vitro compare stability trait. Furthermore, it whether virulence selected strains would be impaired. Exposure stress prior propagation, or vitro, both resulted increasing tolerance. When released, gained lost again production, whereas maintained a high level when EPNs were cultured culture. sp., culture conditions produce highly homozygous, genetically stable inbred lines. investigation provides easily applicable methods heterorhabditid through breeding Compared nematodes from production media yielded higher virulence.