Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis.

摘要: The severe acute respiratory syndrome–associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little known about the load SARS-CoV in oral droplets. We examined specimens, including throat wash and saliva, found large amounts RNA both (9.58 x 10 2 5.93 6 copies/mL) saliva (7.08 103 6.38 108 from all specimens 17 consecutive probable SARS case-patients, supporting possibility transmission Immunofluorescence study showed replication SARSCoV cells derived wash, demonstrating developing a convenient antigen detection assay. This finding, with high rate median 4 days after disease onset before development lung lesions four patients, suggests that should included sample collection guidelines for diagnosis.