Carnosine protects proteins against methylglyoxal-mediated modifications
作者： Alan R. Hipkiss , Harj Chana
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摘要: Abstract Methylglyoxal (MG) (pyruvaldehyde) is an endogenous metabolite which present in increased concentrations diabetics and implicated formation of advanced glycosylation end-products (AGEs) secondary diabetic complications. Carnosine (β-alanyl-L-histidine) normally long-lived tissues at up to 20mM humans. Previous studies showed that carnosine can protect proteins against aldehyde-containing cross-linking agents such as aldose ketose hexose triose sugars, malondialdehyde, the lipid peroxidation product. Here we examine whether protein exposed MG. Our results show readily reacts with MG thereby inhibiting MG-mediated modification revealed electrophoretically. We also investigated could intervene when were MG-induced AGE (i.e. lysine incubated MG). inhibit induced by a lysine-MG-AGE; this suggests second intervention site for emphasizes its potential possible non-toxic modulator
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