Development of a simple screening system for endocrine disruptors.
作者： Eiji Nomura , Ayako Nakajima , Teruo Sugawara
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摘要: BACKGROUND: An endocrine disruptor is a synthetic chemical, which causes adverse effects in an organism, or its progeny, after causing perturbations the system. It important to know chemicals have endocrine-disrupting action. However, increasing number of are being produced by modern chemistry, and examination potential has not yet caught up with advances chemistry. In this study, we developed such screening system for detecting estrogen-like effects. MATERIAL/METHODS: The was based on yeast one-hybrid Both HIS3 lacZ reporter genes connected three tandem copies estrogen response element were prepared. Gal4-estrogen receptor fusion protein made from activation domain (AD) GAL4 transactivator gene then incorporated into plasmid, transfected YM4271 cell strain. effect judged RESULTS: A dual assay-system established transfection both cells. This enabled detection as little 10-12 mol beta-estradiol. CONCLUSIONS: These results show that newly assay useful endocrine-disruptors
参考文章(27)
Anita H. Payne, Christina C. Lawrence, Douglas L. Foster, Robert B. Jaffe, Intranuclear Binding of 17β-Estradiol and Estrone in Female Ovine Pituitaries following Incubation with Estrone Sulfate Journal of Biological Chemistry. ,vol. 248, pp. 1598- 1602 ,(1973) , 10.1016/S0021-9258(19)44232-4
M T Tzukerman, A Esty, D Santiso-Mere, P Danielian, M G Parker, R B Stein, J W Pike, D P McDonnell, Human estrogen receptor transactivational capacity is determined by both cellular and promoter context and mediated by two functionally distinct intramolecular regions. Molecular Endocrinology. ,vol. 8, pp. 21- 30 ,(1994) , 10.1210/MEND.8.1.8152428
Stanley Fields, Ok-kyu Song, A novel genetic system to detect protein-protein interactions. Nature. ,vol. 340, pp. 245- 246 ,(1989) , 10.1038/340245A0
Jun-ichi Nishikawa, Koichi Saito, Jun Goto, Fumi Dakeyama, Masatoshi Matsuo, Tsutomu Nishihara, New Screening Methods for Chemicals with Hormonal Activities Using Interaction of Nuclear Hormone Receptor with Coactivator Toxicology and Applied Pharmacology. ,vol. 154, pp. 76- 83 ,(1999) , 10.1006/TAAP.1998.8557
Kyungsil Yoon, Lea Pallaroni, Matthew Stoner, Kevin Gaido, Stephen Safe, Differential activation of wild-type and variant forms of estrogen receptor α by synthetic and natural estrogenic compounds using a promoter containing three estrogen-responsive elements The Journal of Steroid Biochemistry and Molecular Biology. ,vol. 78, pp. 25- 32 ,(2001) , 10.1016/S0960-0760(01)00070-X
L J Paulozzi, International trends in rates of hypospadias and cryptorchidism. Environmental Health Perspectives. ,vol. 107, pp. 297- 302 ,(1999) , 10.1289/EHP.99107297
ANA M. SOTO, CARLOS SONNENSCHEIN, Cell proliferation of estrogen-sensitive cells: the case for negative control. Endocrine Reviews. ,vol. 8, pp. 44- 52 ,(1987) , 10.1210/EDRV-8-1-44
Charles P. Martucci, Jack Fishman, P450 enzymes of estrogen metabolism Pharmacology & Therapeutics. ,vol. 57, pp. 237- 257 ,(1993) , 10.1016/0163-7258(93)90057-K
William R. Kelce, Christy R. Stone, Susan C. Laws, L. Earl Gray, Jon A. Kemppainen, Elizabeth M. Wilson, Persistent DDT metabolite p,p'-DDE is a potent androgen receptor antagonist. Nature. ,vol. 375, pp. 581- 585 ,(1995) , 10.1038/375581A0
F. VIGNON, M. TERQUI, B. WESTLEY, D. DEROCQ, H. ROCHEFORT, Effects of Plasma Estrogen Sulfates in Mammary Cancer Cells Endocrinology. ,vol. 106, pp. 1079- 1086 ,(1980) , 10.1210/ENDO-106-4-1079