Transforming capacity of two novel genes JS-1 and JS-2 located in chromosome 5p and their overexpression in human esophageal squamous cell carcinoma.

摘要: Esophageal squamous cell carcinoma (ESCC) has a high mortality rate and geographic differences in incidence. Previous studies of comparative genomic hybridization (CGH) showed that chromosomal 5p is frequently amplified lines primary ESCC Hong Kong Chinese origin. In this report, attempt was made to study two novel genes, named as JS-1 JS-2, which are located chromosome 5p15.2 5' upstream delta catenin for their roles molecular pathogenesis ESCC. Eleven lines, 27 cases multiple human tissue cDNA panels (MTC) digestive system were studied the expression level JS-2 by RT-PCR. The full-length sequences determined from non-tumor esophageal epithelial line 3' rapid amplification ends (RACE). transforming capacity also investigated transfecting NIH 3T3 cells with vector pcDNA3.1(-) cloned full coding it followed foci formation transfected under confluence growth anchorage-independent soft agar. Forty-five percent (5/11) 18% (2/11) overexpression respectively, while 55% (15/27) 14% (3/22) respectively. most common patients stage II (6/27; 22%) whereas only overexpressed dysplastic lesion (1/22; 4%) III tumors (2/22; 9%). levels both low normal tissues. Overexpression caused colony agar but not JS-2. A grade sarcoma formed athymic nude mice when overexpressing injected subcutaneously. Our results thus indicate frequent its may play critical role present forms ground work further identification mechanisms carcinogenesis other cancers.