Amplification of cyclin D1 and MDM-2 in oesophageal carcinoma

摘要: Abstract Aims: This study investigated amplification of the cyclin D1 and MDM-2 genes, overexpression gene product, in oesophageal carcinoma. Methods: Paired tumour normal DNA samples from 26 adenocarcinomas 19 squamous cell carcinomas were analysed by Southern blotting with specific probes for , a control ( α-lactalbumin ). The copy numbers calculated each tumour. Expression was assessed immunohistochemical analysis its protein product. Results: Cyclin (by factor between two six) identified seven tumours (16%). 11) 10 (22%). Overexpression eight significantly associated P =0.04; Fisher's exact test), early T stage =0.01; test). Conclusions: occur, although infrequently, development may influence behaviour, causing disease to present at an earlier stage. mechanism this effect is unclear, warrants further investigation.