Amplification and overexpression of the MDM2 gene in a subset of human malignant gliomas without p53 mutations.

摘要: The MDM2 (murine double minute 2) gene has recently been shown to code for a cellular protein that can complex the p53 tumor suppressor product and inhibit its function. We studied series of 157 primary brain tumors report here is amplified overexpressed in 8-10% glioblastomas anaplastic astrocytomas. Thus, represents second most frequently after epidermal growth factor receptor these types. Sequencing transcripts cases with amplification revealed no mutations restriction fragment length polymorphism analysis showed, one exception, losses alleles on chromosome 17. Our results indicate overexpression may be an alternative molecular mechanism by which subset human malignant gliomas escapes from p53-regulated control.