Analyses of human gliomas by restriction landmark genomic scanning.

作者: Mitsutoshi Nakamura , Noboru Konishi , Shigeru Tsunoda , Yoshio Hiasa , Toshihide Tsuzuki

DOI: 10.1023/A:1005712308061

关键词:

摘要: The 16 primary gliomas were examined for changes in genomic DNA using arecently developed 2-dimensional gel electrophoresis method calledrestriction landmark scanning (RLGS). This approach allows detectionof amplification, deletion, methylation and potentially other geneticrearrangements represented as decreases increases spot/fragmentintensity on an autoradiogram. Approximately 2,000 sites tumorDNA compared with those of isolated from normal brain tissues.Seven spots showing intensified signal consistently detected atleast 50% tumors, implying activation corresponding DNAsequences, 8 additional having reduced observed, againin more than all suggesting inactivation by the lossof 1 allele or homozygous deletion. Decreased may also infer relativeCpG island state. Of identified intumors, 2 amplified 4 found to be characteristic oflow- high-grade while remaining 5 reducedspots associated only, a link ofspecific mutations degree malignancy. A separate subset ofglioblastomas evaluated, however, showed no alterations these ’hot spots‘which even low grade astrocytomas. results demonstratethe genetic heterogeneity glioblastoma implicate progression ofneoplasia via differing pathways.

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