Identification of a new breast cancer-related gene by restriction landmark genomic scanning.

摘要: Background: Molecular genetic analyses have demonstrated the importance of accumulation genomic changes in etiology cancer and, additionally, identified valuable markers for certain cancers. Although several prognostic already been breast cancer, it is clear that others remain to be identified. Materials and Methods: Fourteen samples non-cancerous counterparts were applied restriction landmark scanning (RLGS) 6 cell lines (MCF-7, MDA-MB-435, T-47D, MDA-MB-231, SK-BR-3 BT-20) 9 tissue reverse transcriptional polymerase chain reaction (RT-PCR) screening novel alterations. Results: Two spots on RLGS profiles cancerous differed from those normal tissue. Nucleotide sequencing homology search analysis showed these represented voltage-dependent calcium channel ·1H subunit gene (CACNA1H gene) a locus immediately downstream growth factor receptor-binding protein 7 (GRB7) gene. Expression CACNA1H was confirmed by RT-PCR. Conclusion: genes, Grb7 CACNA1H, RLGS. The expression first time. Restriction type two-dimensional electrophoresis employed assessment whole genome (1). performed digesting DNA with enzymes radiolabelling enzyme-derived 5' protruding ends. fragments are separated two rounds visualized autoradiography. An profile typically displays over 2000 radiolabelled sites appear as autoradiograph has three principal advantages analysis. First, allows single assay, whereas other methods, such PCR or Southern blotting, can detect only each assay. appearance disappearance an spot correlated one alteration. unknown sequences. Second, quantitative, intensity signal any representative number