Different patterns of DNA alterations detected by restriction landmark genomic scanning in heterogeneous prostate carcinomas.
作者： N Konishi , Y Hiasa , M Nakamura , Y Kitahori , K Matsubara
DOI:
关键词: Restriction map 、 Restriction landmark genomic scanning 、 Prostate cancer 、 Adenocarcinoma 、 Carcinogenesis 、 Cancer 、 Biology 、 Restriction fragment 、 Pathology 、 Nucleic acid amplification technique
摘要: Alterations in the genomic DNAs of six heterogeneous prostate carcinomas, as well that individual and histologically distinct foci within tumors, were examined using restriction landmark scanning, a method employing two-dimensional gel analysis large number DNA fragments generated by digestion with highly specific endonucleases. Upon autoradiographic imaging, these appear spots varying intensity location for each sample. In our study, comparison cancer against normal controls yielded alterations at least 35 spots. Despite differences histological grading 3 common to all tumor samples showed consistent amplification 8 other demonstrated reduction when compared control. addition, spot occurred between identical isolated from single tumors. We suggest changes detected profiles scanning reflect aberrations yet unidentified oncogenes suppressor genes indicate is not only multifocal but also genetically multicentric.
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