A GENETIC LINKAGE MAP OF THE MOUSE USING RESTRICTION LANDMARK GENOMIC SCANNING (RLGS)

作者: S Hirotsune , M Muramatsu , Y Hayashizaki , Y Okazaki , H Shibata

DOI: 10.1093/GENETICS/138.4.1207

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摘要: We have developed a multiplex method of genome analysis, restriction landmark genomic scanning (RLGS) that has been used to construct genetic maps in mice. Restriction landmarks are end-labeled fragments DNA separated by using high resolution, two-dimensional gel electrophoresis identifying as many two thousand loci single gel. Variation for several hundred these identified between laboratory strains and Mus spretus. The segregation more than 1100 RLGS analyzed recombinant inbred (RI) separate interspecific crosses. Genetic derived link 1045 reference on all the autosomes X chromosome mouse genome. can be applied analysis different organisms identify because it uses end-labeling rather probe hybridization. Different combinations enzymes yield sets providing expanded power mapping.

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