RLCS, Restriction Landmark cDNA Scanning

摘要: It is important to identify and isolate differentially expressed genes whose expression underlies many biological processes such as development, differentiation, cellular response various stimuli, also pathological changes that arise in diseases. Classically, two hybridization methods, differential subtractive hybridization, have been used analyze [1–3]. However, effective only for mRNAs abundantly one of the types cells. Subtractive rather empirical poor reproducibility. Furthermore, both methods are time-consuming. Recently, Liang Pardee developed a novel method called display using an arbitrarily primed reverse transcription-coupled PCR sequencing gels [4]. This has proved be powerful technique detecting eukaryotic gene expression; it simple, sensitive, time-saving. PCR-mediated amplification with arbitrary primers requires delicate control, depending on [5,6], cDNA probes thus obtained often give false positive signals Northern blot analysis [7,8]. seems less sensitive rare mRNA populations, since strong bias toward higher abundance [9].