Imunmoglobulin light-chain immunoblots of urine proteins from patients with tubular and Bence-Jones proteinuria
作者： Anthony G.W. Norden , Leah M. Fulcher , Frederick V. Flynn
DOI: 10.1016/0009-8981(87)90434-7
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摘要: Abstract Immunoglobulin excretion by patients with monoclonal gammopathies and tubular proteinuria has been analysed agarose gel isoelectricfocussing of untreated urine immunoblotting. About three-quarters the Bence-Jones proteins detected occurred as multiple bands on isoelectricfocussing; in about half these cases forms were due to polymerisation or fragmentation light chains. In specimens proteinuria, a characteristic chain pattern three broad covering p I ranges 7.1–7.3, 7.8–8.0 8.3–8.5 was found. This also 53% identical that found concentrated normal urine. Intact immunoglobulin usually appeared more evenly spaced similar intensity whereas polyclonal intact immunoglobulins produced diffuse staining from 5.0–8.5. A scheme for qualitative analysis urinary using this technique developed.
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