Apolipoprotein A-I expression in rats is not altered by troglitazone.

摘要: Insulin is known to upregulate apolipoprotein A-I (apoA-I) promoter activity and increase apoA1 gene expression in vivo. To determine if enhancement of insulin action with sensitizers can also the apoA-I expression, we studied vivo effect troglitazone, a potent sensitizer, on rat hepatic intestinal mRNA using Northern blot analysis. The plasma, hepatic, content was measured immunoblot analysis specific anti-rat antiserum. Troglitazone, given mixed chow (0.2%) for 18 days, did not either plasma or tissue protein content. Intestinal relative glyceraldehyde-3 phosphate dehydrogenase (G(3)PDH) significantly lower compared both control troglitazone-treated rats. troglitazone examined transient transfection HepG2 cells transfected apoA-I-chloramphenicol acetyl transferase (CAT) reporter plasmid (pAI.474.CAT). CAT (percentage acetylation chloramphenicol as means +/- SEM) different ethanol (vehicle)-treated treated (50.5% 2.5% vs 57.7% 8.2% 53.5% 4.2% 10 100 mM respectively). It concluded that doses achieve sensitization enhance sufficiently result an increased intact rat. However, peroxisome proliferator activator receptor (PPAR) agonists have significant PPAR alpha addition their gamma effects, may well be able induce expression.