Analysis of the interference of polyphenols and proanthocyanidins on the neutral red assay for cell viability.
作者： Maitree Suttajit , Wandee Wattanachaiyingcharoen , Robert W. Gracy , Puksiri Sinchaiyakit , Surisak Prasarnpun
DOI:
关键词:
摘要: The neutral red uptake assay has been wildly used for screening cell viability. However, several natural polyol compounds such as polyphenolics and flavonoids have observed to interfere with the give false positive results. This study was designed determine whether polyphenolic proanthocyanidins extracted from tamarind seed husk (TSH) affect Two kinds of normal cells, retina ganglion cells (RGC-5) hippocampal (HT-22), were in study. It found that by using assay, TSH extract increased viability maximum level 2-folds higher than control. artifact similarly shown both serum-free serum-added media which had no proliferation. Therefore, may not suitably survival test especially presence or materials containing polyphenols proanthocyanidins. Keywords: Polyphenol, proanthocyanidins, viability, assay.
nu.ac.th参考文章(14)
Puksiri Sinchaiyakit, Yohji Ezure, Sarin Sriprang, Supakorn Pongbangpho, Nasapon Povichit, Maitree Suttajit, Tannins of tamarind seed husk: preparation, structural characterization, and antioxidant activities. Natural Product Communications. ,vol. 6, pp. 829- 834 ,(2011) , 10.1177/1934578X1100600619
Y. Sudjaroen, R. Haubner, G. Würtele, W.E. Hull, G. Erben, B. Spiegelhalder, S. Changbumrung, H. Bartsch, R.W. Owen, Isolation and structure elucidation of phenolic antioxidants from Tamarind (Tamarindus indica L.) seeds and pericarp Food and Chemical Toxicology. ,vol. 43, pp. 1673- 1682 ,(2005) , 10.1016/J.FCT.2005.05.013
P. Olivier, P. Testard, D. Marzin, D. Abbott, Effect of high polyol concentrations on the neutral red absorption assay and tetrazolium-MTT test of rat hepatocytes in primary culture Toxicology in Vitro. ,vol. 9, pp. 133- 138 ,(1995) , 10.1016/0887-2333(94)00209-D
K Chiba, K Kawakami, K Tohyama, Simultaneous evaluation of cell viability by neutral red, MTT and crystal violet staining assays of the same cells. Toxicology in Vitro. ,vol. 12, pp. 251- 258 ,(1998) , 10.1016/S0887-2333(97)00107-0
Sanjoy Roychowdhury, Gerald Wolf, Gerburg Keilhoff, Debasis Bagchi, Thomas Horn, Protection of primary glial cells by grape seed proanthocyanidin extract against nitrosative/oxidative stress. Nitric Oxide. ,vol. 5, pp. 137- 149 ,(2001) , 10.1006/NIOX.2001.0335
Ann E. Hagerman, Extraction of tannin from fresh and preserved leaves. Journal of Chemical Ecology. ,vol. 14, pp. 453- 461 ,(1988) , 10.1007/BF01013897
Filipe Miguel Areias, A.Cristina Rego, Catarina R Oliveira, Rosa Maria Seabra, Antioxidant effect of flavonoids after ascorbate/Fe2+-induced oxidative stress in cultured retinal cells Biochemical Pharmacology. ,vol. 62, pp. 111- 118 ,(2001) , 10.1016/S0006-2952(01)00621-9
P. Siddhuraju, Antioxidant activity of polyphenolic compounds extracted from defatted raw and dry heated Tamarindus indica seed coat Lwt - Food Science and Technology. ,vol. 40, pp. 982- 990 ,(2007) , 10.1016/J.LWT.2006.07.010
Joungil Choi, Craig C Conrad, Christina A Malakowsky, John M Talent, Chun-Su Yuan, Robert W Gracy, Flavones from Scutellaria baicalensis georgi attenuate apoptosis and protein oxidation in neuronal cell lines Biochimica et Biophysica Acta. ,vol. 1571, pp. 201- 210 ,(2002) , 10.1016/S0304-4165(02)00217-9
Kimberly N. Wisman, Akeysha A. Perkins, Melanie D. Jeffers, Ann E. Hagerman, Accurate assessment of the bioactivities of redox-active polyphenolics in cell culture. Journal of Agricultural and Food Chemistry. ,vol. 56, pp. 7831- 7837 ,(2008) , 10.1021/JF8011954