Quantitative kinetic analysis of β-glucuronidase activities using a computer-directed microtiter plate reader
作者： Peter Breyne , Marc De Loose , Andrée Dedonder , Marc Van Montagu , Ann Depicker
DOI: 10.1007/BF02670556
关键词:
摘要: We have established a procedure for automated, kinetic analysis of β-glucuronidase (GUS) activities using colorimetric or fluorometric microtiter plate reader connected to computer that directs the measurements and accesses data. Compared with end-point measurements, saves time, is more accurate, needs 20 times less material. It allows precise determination GUS over range 400,000-fold, limit detection about 0.01 units per mL in assay 0.1 milliunit assay. A general protocol transgenic plant tissue was worked out applied investigate expression chimeric gene stably transformed tobacco calli.
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sci-hub.se 参考文章(9)
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