ABCG2 is a potential marker of tumor-initiating cells in breast cancer

作者: Renata Danielle Sicchieri , Willian Abraham Da Silveira , Larissa Raquel Mouro Mandarano , Tatiane Mendes Goncalves de Oliveira , Hélio Humberto Angotti Carrara

DOI: 10.1007/S13277-015-3647-0

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摘要: The existence of tumor-initiating cells (TICs) within solid tumors has been hypothesized to explain tumor heterogeneity and resistance cancer therapy. In breast cancer, the expression CD44 CD24 activity aldehyde dehydrogenase 1 (ALDH1) can be used selectively isolate a cell population enriched in TICs. However, ideal marker identify TICs not established. aim this study was evaluate novel potential markers for TIC carcinoma. We prospectively analyzed CD44, CD24, ABCG2, CXCR4, ALDH1 by using flow cytometry 48 invasive ductal carcinomas from locally advanced metastatic patients who were administered primary chemotherapy. A mammosphere assay employed 30 samples. relationship among cytometric analyses, ABCG2 gene expression, clinical pathological responses therapy analyzed. GSE32646 database silico genes associated with low high expression. observed that presence ABCG2+ only predict formation mammospheres vitro (R 2 = 0.15, p = 0.029). Quantitative polymerase chain reaction (qPCR) revealed positive correlation between tumor. predictive response neoadjuvant chemotherapy our experiments dataset (p = 0.04 p = 0.002, respectively). analysis demonstrated ABCG2Up samples have slower cycle higher membrane proteins but greater chromosomal instability, metastasis, immune evasion, hypoxia. Such genetic characteristics are compatible highly aggressive resistant tumors. Our results support hypothesis is chemotherapy, based on assays profile, we show, first time, protein as an independent identification cancer.

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