Production of enterotoxins of Staphylococcus spp. isolated from samples of sheep milk
作者： František Zigo , Milan Vasiľ , Juraj Elečko , Martin Lapin , Zuzana Farkašova
DOI: 10.5219/361
关键词:
摘要: In our study was followed occurrence of mastitis in herd 430 sheep breed zoslachtena valaska with hand milking technology examined two times during one lactation season. Individual examination consisted from clinical udder and microbiological milk samples. By PCR determined presence genes coding production enterotoxins, by ELISA methods individual types enterotoxins. From forms were frequently detected subacute (6.7%), subclinical (5.7%) acute (2.9%). The coagulase-negative staphylococci (CNS) identified 102 (65.4%) all 156 positive isolates. CNS and S. aureus caused (5.1%), (3.9%) (2.4%) mastitis. most isolated were S. epidermidis, by S. chromogenes and S. xylosus from ewes chronical were predominantly isolated S. aureus, S. uberis epidermidis. staphylococcal enterotoxins (SE) - SEA, SEB, SEC, SED the genes sec (3), sea (2), seb (2) and sed (2) in S. epidermidis, S. schleiferi and S. chromogenes, respectively. results suggested on high (12.4%) forms. Confirmed their present a risk for human health decreased quality products sheep´s milk.
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