Genetic mosaic with dual binary transcriptional systems in Drosophila.

摘要: MARCM (mosaic analysis with a repressible cell marker) involves specific labeling of GAL80-minus and GAL4-positive homozygous cells in otherwise heterozygous tissues. Here we demonstrate how the concurrent use two independent binary transcriptional systems may facilitate complex studies Drosophila nervous system. By fusing LexA VP16 acidic activation domain (VP16) or GAL4 (GAD), obtained both GAL80-insensitive GAL80-suppressible factors. LexA::VP16 can mediate MARCM-independent transgene induction mosaic organisms. The incorporation LexA::GAD into MARCM, which call dual-expression-control permits distinct transgenes different patterns among Lineage suggested presence neuroglioblasts developing optic lobes but did not indicate production glia by postembryonic mushroom body neuronal precursors. In addition, ubiquitous driver revealed many unidentified GAL4-GH146-positive projection neuron lineages.