High-performance liquid chromatographic separation of peptides on a diol-Gly-Phe-Phe tripeptide-bonded phase.
作者： Thomas C. Pinkerton , Kenneth A. Koeplinger
DOI: 10.1016/S0021-9673(00)90559-4
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摘要: The retention characteristics of some selected peptides (mol. wt. less than 2000 a.m.u.) have been investigated on a diol-Gly-Phe-Phe partitioning phase, bound to 5-microns porous silica. hydrophobic, positively charged can be separated with mild mobile phases, containing only acetonitrile and phosphate buffer. peptide selectivity the diol-Gly-Phe-Phe-bonded phase is uniquely different from that C8 column. dependence capacity factors pH, ionic strength, organic solvent concentration demonstrated mechanisms involve multifunctional reversed-phase cation-exchange processes.
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