High-performance liquid chromatography packing materials for the analysis of small molecules in biological matrices by direct injection.

摘要: The increasing demand on high-performance liquid chromatography to resolve mixtures of closely related components in complex biological matrices less time with higher precision has led the development a variety new columns, which eliminate need for sample preparation. These packings isolate small molecules from macromolecules direct injection by exerting two separation mechanisms. They allow elution all high recovery one peak at extraparticulate void, because size-exclusion interactions hydrophilic outer particulate surfaces. Simultaneously, these permeation and partitioning bonded-phases are protected contamination macromolecules. names given include "internal surface reversed-phase", "shielded hydrophobic phase", "semipermeable surface", "dual zone material" "mixed-functional phases". fundamental principles behind each design concepts reviewed, applications cited.