Chromosome Replication and the Division Cycle of Escherichia coli B/r

作者: H. E. Kubitschek , M. L. Freedman

DOI: 10.1128/JB.107.1.95-99.1971

关键词:

摘要: The average amount of deoxyribonucleic acid (DNA) per cell was measured in steady-state cultures Escherichia coli B/r grown at 37 C glucose-limited chemostats or batch the exponential growth phase as maintained with one several carbon sources. Within experimental errors, DNA content dependent only on rate and independent type culture, source, addition factors. increased continuously over range 0.02 to 3 divisions hour. data entire rates are agreement a constant time for single replication point traverse genome, 47 min, division following 25 min after termination replication. genome 4.2 x 10(-15) g (or 2.5 10(9) daltons).

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