Dissociation of catalytic activity and neurotoxicity of a basic phospholipase A2 from Russell's viper (Vipera russelli) venom
作者： G.P. Jayanthi , S. Kasturi , T.Veerabasappa Gowda
DOI: 10.1016/0041-0101(89)90099-8
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摘要: Abstract G. P. Jayanthi , S. Kasturi and T. V. Gowda . Dissociation of catalytic activity neurotoxicity a basic phospholipase A 2 from Russell's viper ( Vipera russelli ) venom. Toxicon 27, 875–885, 1989.—A neurotoxic VRV PL-V was purified venom in single step by CM-Sephadex C-25 column chromatography. is PLA with mol. wt approximately 10,000. The lethal potency greater than that the crude showed anticoagulant induced edema foot pad mouse. undergoes aggregation at pH 4.8. size aggregate increased as temperature which enzyme incubated raised. highly aggregated form 53,100 formed 96°C. This two-fold increase its activity, while disappeared. also significant when compared to monomeric form. Edema-inducing decreased upon association higher molecular
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