Effects of adenoviral-mediated coexpression of bone morphogenetic protein-7 and insulin-like growth factor-1 on human periodontal ligament cells.

摘要: Yang L, Zhang Y, Dong R, Peng Liu X, Wang Cheng X. Effects of adenoviral-mediated coexpression bone morphogenetic protein-7 and insulin-like growth factor-1 on human periodontal ligament cells. J Periodont Res 2010; 45: 532–540. © 2010 John Wiley & Sons A/S Background Objective:  Bone (BMP-7) (IGF-1) are important in reconstruction. However, their synergistic effect regeneration by gene delivery has not been reported. In this study, these two factors to cells (hPDLCs) was examined for its effects cell proliferation differentiation. Material Methods:  Recombinant adenoviruses containing both BMP-7 IGF-1 cDNA created introducing the internal ribosome entry site (IRES) sequence were used transfer genes into hPDLCs. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay cycle analysis observe proliferation, while alkaline phosphatase activity measurement, RT-PCR vivo tests conducted investigate differentiation. Results:  The hPDLCs transduced coexpressing suppressed differentiation ability enhanced. There a synergism up-regulating mRNA levels collagen type I Runx2. Implantation with scaffolds illustrated that exhibited osteogenic formed bone-like structures. Conclusion:  combined using an IRES-based strategy synergistically enhanced It is suggested could be new potential method therapy