Rapid purification of extracellular tannase using polyethylene glycol–tannic acid complex

摘要: This paper reports on a rapid procedure for partial purification of extracellular tannase using combination tannic acid and PEG-6000. A pH-dependent protein precipitation was obtained within 30 min. Monitoring the primary curve culture filtrate at varying pH, second step designed that yielded an approximately eight-fold increase in enzyme purification. is importance as no temperature pH monitoring required very fast without any alteration enzymatic properties precipitant.