Role of Monocyte Chemotactic Protein-1/CC Chemokine Ligand 2 on γδ T Lymphocyte Trafficking during Inflammation Induced by Lipopolysaccharide or Mycobacterium bovis Bacille Calmette-Guérin

摘要: γδ T lymphocytes are involved in a great variety of inflammatory and infectious responses. However, the mechanisms by which migrate to inflamed sites poorly understood. In this study we investigate role monocyte chemotactic protein (MCP)-1 regulating cell migration after LPS or Mycobacterium bovis bacille Calmette-Guerin (BCG) challenge. LPS-induced influx was significantly inhibited either pretreatment with dexamethasone vaccinia virus Lister 35-kDa chemokine binding protein, vCKBP, CC neutralizing suggesting for chemokines phenomenon. stimulation increased expression MCP-1 mRNA at inflammation site within 6 h. It is noteworthy that unable increase production recruitment C3H/HeJ, indicative involvement Toll-like receptor 4. cells express CCR2. Pretreatment anti-MCP-1 mAb drastically vivo mobilization. Indeed, knockout mice were recruit pleural cavity stimulation, effect could be restored coadministration MCP-1. addition, BCG-induced lymphocyte accumulation reduced when compared wild-type mice. conclusion, our results indicate dependent on 4 sensitive both chemokine-binding inhibition. Moreover, using Abs genetically deficient show LPS- mainly orchestrated