Mechanical property characterization of hundreds of single nuclei based on microfluidic constriction channel.

摘要: As label-free biomarkers, the mechanical properties of nuclei are widely treated as promising biomechanical markers for cell type classification and cellular status evaluation. However, previously reported parameters were derived from only around 10 nuclei, lacking statistical significances due to low sample numbers. To address this issue, first isolated SW620 A549 cells, respectively, using a chemical treatment method. This was followed by aspirating them through two types microfluidic constriction channels property characterization. In study, hundreds characterized, producing passage times 0.5 ± 1.2 s in I channel (n = 153), 0.045 0.047 II 215) 0.50 0.86 channel. addition, neural network based pattern recognition used classify successful rates 87.2% diameters 85.5% 89.3% both nuclei. These results indicate that characterization may contribute different tumor cells.