Dynamic, mechanical integration between nucleus and cell- where physics meets biology

摘要: Nuclear motions like rotation, translation and deformation suggest that the nucleus is acted upon by mechanical forces. Molecular linkages with cytoskeleton are thought to transfer forces nuclear surface. We developed an approach apply reproducible, known in spread adherent cells quantified elastic response of mechanically integrated nucleus-cell. The method sensitive molecular perturbations revealed new insight into function LINC complex. While these experiments behaviors, turnover assembly/disassembly binding/unbinding expected dissipate any stored energy or cytoskeleton. Experiments investigating over longer time scales demonstrated principle expansive/compressive stresses on surface arise from movement cell boundaries shape position nucleus. Such can conform shapes during movements without myosin activity.