Human brain in tissue culture
作者： Donald H. Gilden , Zofia Wrobłewska , Lawrence F. Eng , Lucy Balian Rorke
DOI: 10.1016/0022-510X(76)90169-6
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摘要: Glial fibrillary acidic protein (GFAP) was present in cell cultures derived from human fetal brain tissue as determined by indirect immunofluorescence (IF) and immunoperoxidase (IP) staining using rabbit anti-human GFAP antisera. The IF IP techniques were comparable localizing the cytoplasmic distribution frequent perinuclear concentration of cells. horseradish peroxidase technique more sensitive a 1:20–1:40 dilution anti-GFAP serum could be applied initial step compared to 1:10 for staining. Sequential studies subcultivated lines these indicated that some become GFAP-negative rapidly, whereas other remain GFAP-posifive no less than ten subcultivations vitro. GFA never any PMLSV40-transformed
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