Studies on the clonal origin of human B cell leukemia using monoclonal anti-idiotype antibodies.

摘要: The clonal origin of an IgA1 kappa B cell leukemia in a 71-year-old man (WF) was examined using monoclonal anti-Id antibody and panel anti-VH antibodies. Immunofluorescent studies revealed that all surface + leukemic cells WF's blood 10% the IgM+ his bone marrow expressed WF Id. Three percent also gamma-chains their cytoplasm. Approximately 0.1%, 1%, mononuclear cells, respectively, mu-chains, gamma-chains, alpha-chains cytoplasm, but no detectable light chains or immunoglobulins. These mu, gamma, alpha-positive had convoluted nucleus narrow cytoplasm characteristic normal mu+ pre-B cells. Sequential isotype switching among this unusual population indicated by co-expression mu-chains 11% 63%, gamma cytoplasmic were reactive with one four data suggest malignant transformation clone before switching, imply chain precommitment at level differentiation.