Expression of an Extracellular Calcium-Sensing Receptor in Human and Mouse Bone Marrow Cells

摘要: The cloning of a G protein–coupled, extracellular calcium (Ca2+e)-sensing receptor (CaR) from bovine parathyroid provided direct evidence that Ca2+e-sensing can occur through receptor-mediated activation proteins and their associated downstream regulators cellular function. CaR transcripts protein are present in various tissues humans other mammals involved Ca2+e homeostasis, including parathyroid, kidney, thyroidal C-cells. study was performed to determine whether bone marrow cells express the CaR, since within space could be exposed substantial changes related turnover. Using DNA RNA probes human cDNA, we identified 5.2 ∼4.0 kilobases by Northern analysis poly(A+) low-density mononuclear isolated whole putatively enriched progenitor cells, cell precursors. In situ hybridization also same preparations. Reverse transcription-polymerase chain reaction demonstrated >99% nucleotide identity between corresponding regions cDNA. Antisera specific for several different domain were reactive with either adherent or nonadherent plastic. About one-third adherent, CaR-immunoreactive positive alkaline phosphatase, nonspecific marker preosteoblasts, osteoblasts, assorted colony-forming unit-fibroblast lineage. addition, fraction (∼60%) low density murine cultured 1 week at 4.8 mM expressed both immunoreactivity esterase, an enzyme monocyte/macrophages fibroblasts. Finally, erythroid precursors megakaryocytes as well blood platelets abundant immunoreactivity, while peripheral erythrocytes most polymorphonuclear leukocytes did not. These studies indicate is hematopoietic lineages potentially play role controlling function types space.